About
Research scientist at UCL Institute of Ophthalmology, working on membrane trafficking, melanosome biogenesis and photoreceptor development and renewal.
Research INTERESTS
Research Associate - University College London
April 2011 – Present
April 2011 – Present
Investigating OA1 cellular trafficking with the aim to better understand its role in melanosome biogenesis. Rhodopsin transport and rod photoreceptor disc renewal. The relationship between EGF receptor and melansomal precursor protein trafficking. Retinal pigment epithelium cell phagocytosis and basal infolding structural analysis. Studying age related disease using Human iPS-derived retinal pigment epithelium cells as a model.
Ciliary Function Scientist - Royal Brompton Hospital
January 2009 – March 2011
January 2009 – March 2011
Electron tomography analysis of classical primary ciliary dyskinesia (PCD) defects and characterising novel ciliary ultrastructural defects present in difficult to diagnose patients. Determining if chlamydomonas make a suitable model organism to study human PCD by comparing flagella and cilia ultrastructural.
PhD - Imperial College London
September 2005 – September 2008
September 2005 – September 2008
Using electron tomography to generate 3D reconstructions and model cardiac muscle I-band and Z-band as well as precisely determining the range of thin filament lengths found in cardiac muscle.
Publications
2015
Burgoyne T, Meschede IP, Burden JJ, Bailly M, Seabra MC, Futter CE. (2015) Rod disc renewal occurs by evagination of the ciliary plasma membrane that makes cadherin-based contacts with the inner segment. Proc Natl Acad Sci U S A. Dec 14Burgoyne T, Morris EP, Luther PK. (2015) Three-dimensional structure of vertebrate muscle Z-band: the small-square lattice Z-band in rat cardiac muscle. Journal of Molecular Biology. 30(5-6): 171–185.Georgiannakis A, Burgoyne T, Lueck K, Futter C, Greenwood J, Moss SE. (2015) Retinal Pigment Epithelial Cells Mitigate the Effects of Complement Attack by Endocytosis of C5b-9. J Immunol. 195(7):3382-9.Tomas A, Vaughan SO, Burgoyne T, Sorkin A, Hartley JA, Hochhauser D, Futter CE. (2015) WASH and Tsg101/ALIX-dependent diversion of stress-internalised EGFR from the canonical endocytic pathway. Nat Commun. 6:7324.Burgoyne T, O'Connor MN, Seabra MC, Cutler DF, Futter CE. (2015) Regulation of melanosome number, shape and movement in the zebrafish retinal pigment epithelium by OA1 and PMEL. J Cell Sci. 128(7):1400-7.Burgoyne T, Patel S, Eden ER. (2015) Calcium signaling at ER membrane contact sites. Biochim Biophys Acta. 2015 S0167-4889(15).2014
Hudson N, Powner MB, Sarker MH, Burgoyne T, Campbell M, Ockrim ZK, Martinelli R, Futter CE, Grant MB, Fraser PA, Shima DT, Greenwood J, Turowski P. (2014) Differential apicobasal VEGF signaling at vascular blood-neural barriers. Dev Cell. 30(5):541-52Hjeij R, Onoufriadis A, Watson CM, Slagle CE, Klena NT, Dougherty GW, Kurkowiak M, Loges NT, Diggle CP, Morante NF, Gabriel GC, Lemke KL, Li Y, Pennekamp P, Menchen T, Konert F, Marthin JK, Mans DA, Letteboer SJ, Werner C, Burgoyne T, Westermann C, Rutman A, Carr IM, O'Callaghan C, Moya E, Chung EM; UK10K Consortium, Sheridan E, Nielsen KG, Roepman R, Bartscherer K, Burdine RD, Lo CW, Omran H, Mitchison HM. (2014) CCDC151 mutations cause primary ciliary dyskinesia by disruption of the outer dynein arm docking complex formation. Am J Hum Genet. 95(3):257-74.Burgoyne T, Lewis A, Dewar A, Luther P, Hogg C, Shoemark A, Dixon M. (2014) Characterizing the Ultrastructure of Primary Ciliary Dyskinesia transposition defect using Electron Tomography. Cytoskeleton. 71(5):294-301.Onoufriadis A, Shoemark A, Schmidts M, Patel M, Jimenez G, Liu H, Thomas B, Dixon M, Hirst RA, Rutman A, Burgoyne T, Williams C, Scully J, Bolard F, Lafitte JJ, Beales PL, Hogg C, Yang P, Chung EM, Emes RD, O'Callaghan C; UK10K, Bouvagnet P, Mitchison HM. (2014) Targeted NGS gene panel identifies mutations in RSPH1 causing primary ciliary dyskinesia and a common mechanism for ciliary central-pair agenesis due to radial spoke defects. Hum Mol Genet. 23(13):3362-74.2013
Burgoyne T, Jolly R, Martin-Martin B, Seabra MC, Piccirillo R, Schiaffino MV, Futter CE. (2013) Expression of OA1 limits the fusion of a subset of MVBs with lysosomes; a mechanism likely involved in the initial biogenesis of melanosomes. J Cell Sci. 126(Pt 22):5143-52.Shoemark A, Ives A, Becker-Heck A, Burgoyne T, Dixon M, Bilton D, Wilson R, Omran H, Hogg C. (2013) Inner Dynein Arm Defects in Primary Ciliary Dyskinesia. J Genet Syndr Gene Ther. 4:7.Martins T, Burgoyne T, Kenny BA, Hudson N, Futter CE, Ambrósio AF, Silva AP, Greenwood J, Turowski P. (2013)Methamphetamine-induced nitric oxide promotes vesicular transport in blood-brain barrier endothelial cells. Neuropharmacology. 65:74-82.2012
Eden ER, Burgoyne T, Edgar JR, Sorkin A, Futter CE. (2012) The relationship between ER-multivesicular body membrane contacts and the ESCRT machinery. Biochem Soc Trans. 40(2):464-8Olbrich H, Schmidts M, Werner C, Onoufriadis A, Loges NT, Raidt J, Banki NF, Shoemark A, Burgoyne T, Al Turki S, Hurles ME; UK10K Consortium, Köhler G, Schroeder J, Nürnberg G, Nürnberg P, Chung EM, Reinhardt R, Marthin JK, Nielsen KG, Mitchison HM, Omran H. (2012) Recessive HYDIN mutations cause primary ciliary dyskinesia without randomization of left-right body asymmetry. Am J Hum Genet. 91(4):672-84.Burgoyne T, Dixon M, Luther P, Hogg C, Shoemark A. (2012) Generation of a three-dimensional ultrastructural model of human respiratory cilia. Am J Respir Cell Mol Biol. 47(6):800-6.2008
Burgoyne T, Muhamad F, Luther PK. (2008) Visualization of cardiac muscle thin filaments and measurement of heir lengths by electron tomography. Cardiovasc Res. 77(4):707-12.
